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High Speed Gene Sequencing, using True Single Molecules Sequencing (tSMS)

Hey guys, Check this out! 

New way for gene sequencing and it was design for speed. They're using SOLiD 3 system, and will have more improvements. 

The method is called tSMS, True Single Molecules Sequencing

1. tSMS using single strand 100-200 nt strand, and generate the TAAA tails with a fluorescence A end. These strand served as template. 
2. Then these templates will be hybridize to a flow cell, that treated with billions of helico-T capture site.
3. After hybridization, using laser to illuminate the successful hybridize strand. They then using CCD camera to capture the illumination. 
4. Then, the fluorescence A will be washed away. 
5. Now being the repentance process of adding fluorescence nucleotides A,C,G,T (one at a time) and continue the process of binding, illuminate, visual capturing, and adding next nucleotides again and again, until the end.

It was and article from Wired Science at 28 Oct. Just able to read them recently. 
>.<  I've being outdated! 

Check here. 

or Watch the video for more clarity, as my explanation is very sucks most of the times, kekeke..

Enjoy the video. 


  1. good, cool. some humble opinions and sharing from ck:-

    SOLiD and other approaches like solexa, 454, HeliScope are known as secondary generation DNA sequencing technology. Sequencing approach different as compare with conventional sanger method, whereby this new approach will increase parallelism (i.e. ability to run many reads at one time, it can come to million at a time, rather than few hundred in capillary sequencing)

    secondary generation sequencing technique also allow sequencing on site (i.e. in a solid bead/ mrcochip), and they are collectively know as cyclic array sequencing, like what you mention in your blog, will add one base(with reverse terminator/ blocker),so additional base cant add in, fluorescent scan, then remove away terminator and follow by next base and keep on.

    another different is in the preparation of DNA library, instead of cloning DNA framents to plasmid and later transform to e.coli; the new approach rely on in vitro method, DNA frament attach by common adapter,like TAAA tails u mentiond,

    a major drawback is even it can perform many sequencing reads, but it only handle short read, means the length of sequence read only about 35bp, far shorter than sanger automated sequencing that gv 1000bp read length..which mean at the moment, it could be a good choice for resequencing.

    anyway, eventually scientist can overcome this weakness, just like the traditional method also spent 30 yrs to achieve current performance

    majority of the second generation sequencing techniques base on extension by polymerase, but funny thing is this SOLiD approach utilize ligase.

    I came accross recently about these tech, but only familiar with 454 and solexa, others still digesting...and have few literatures to follow..anyway the follwing literature give a comprehensive review.. for more info or animation presentation, can visit the manufacturer website directly:-)

    for more info, feel free refer the following article:

    Jay Shendure & Hanlee Ji. Next-generation DNA sequencing. Nature Biotechnology 26, 1135 - 1145 (2008)
    Published online: 9 October 2008 doi:10.1038/nbt1486

  2. Wow, choon kiat, haha, Thanks for the elaboration.
    They truly very interesting, haha


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